Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- M00334-7 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Caspase-3 Antibody Picoband™ (monoclonal, 15G8)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Caspase-3 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 15G8
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Submitted references CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling.
Organotin benzohydroxamate derivatives (OTBH) target colchicine-binding site exerting potent antitumor activity both in vitro and vivo revealed by quantitative proteomic analysis.
miR-466 Contributes to the Enhanced Antitumor Effect of Bortezomib on Non-Small-Cell Lung Cancer by Inhibiting CCND1.
Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.
Cryptotanshinone ameliorates MPP(+)-induced oxidative stress and apoptosis of SH-SY5Y neuroblastoma cells: the role of STAT3 in Parkinson's disease.
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Investigation of Lonicera japonica Flos against Nonalcoholic Fatty Liver Disease Using Network Integration and Experimental Validation.
Low expression of miR-1 promotes osteogenic repair of bone marrow mesenchymal stem cells by targeting TLR1.
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.
RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes.
Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.
Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells.
Lei S, Du X, Tan K, He X, Zhu Y, Zhao S, Yang Z, Dou G
Experimental and therapeutic medicine 2023 Jul;26(1):314
Experimental and therapeutic medicine 2023 Jul;26(1):314
Organotin benzohydroxamate derivatives (OTBH) target colchicine-binding site exerting potent antitumor activity both in vitro and vivo revealed by quantitative proteomic analysis.
Song J, Liu S, Ren Y, Zhang X, Zhao B, Wang X, Li Y
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2023 Aug 1;187:106488
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2023 Aug 1;187:106488
miR-466 Contributes to the Enhanced Antitumor Effect of Bortezomib on Non-Small-Cell Lung Cancer by Inhibiting CCND1.
Wang WH, Zhan JM, Tang YL, Zhou N, Liu WY, Jiang DW
Chemotherapy 2022;67(2):110-122
Chemotherapy 2022;67(2):110-122
Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways.
Zhang Q, Qian D, Tang DD, Liu J, Wang LY, Chen W, Wu CJ, Peng W
Journal of agricultural and food chemistry 2022 Sep 7;70(35):10782-10793
Journal of agricultural and food chemistry 2022 Sep 7;70(35):10782-10793
Cryptotanshinone ameliorates MPP(+)-induced oxidative stress and apoptosis of SH-SY5Y neuroblastoma cells: the role of STAT3 in Parkinson's disease.
Wang Q, Liu Y
Metabolic brain disease 2022 Jun;37(5):1477-1485
Metabolic brain disease 2022 Jun;37(5):1477-1485
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Sun M, Zhou D, Wu J, Zhou J, Xu J
Marine drugs 2022 Feb 5;20(2)
Marine drugs 2022 Feb 5;20(2)
Investigation of Lonicera japonica Flos against Nonalcoholic Fatty Liver Disease Using Network Integration and Experimental Validation.
Sun CY, Zhao P, Yan PZ, Li J, Zhao DS
Medicina (Kaunas, Lithuania) 2022 Aug 30;58(9)
Medicina (Kaunas, Lithuania) 2022 Aug 30;58(9)
Low expression of miR-1 promotes osteogenic repair of bone marrow mesenchymal stem cells by targeting TLR1.
Wang JG, Zhao XG, Wang XL, Liu MX, Wan W
European review for medical and pharmacological sciences 2020 Apr;24(7):3492-3500
European review for medical and pharmacological sciences 2020 Apr;24(7):3492-3500
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.
Huang KF, Huang XP, Xiao GQ, Yang HY, Lin JS, Diao Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 May;68(4):455-61
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2014 May;68(4):455-61
RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.
Fu L, Zhang S
International journal of molecular medicine 2014 May;33(5):1153-60
International journal of molecular medicine 2014 May;33(5):1153-60
Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts.
Dong HY, Wang ZF
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2014 Apr;26(2):200-7
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2014 Apr;26(2):200-7
Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes.
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L
Neural regeneration research 2014 Apr 15;9(8):837-44
Neural regeneration research 2014 Apr 15;9(8):837-44
Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs.
Guan YQ, Li Z, Chen J, Tao H, Wang W, Zheng Z, Li L, Liu JM
Biomaterials 2011 May;32(14):3637-46
Biomaterials 2011 May;32(14):3637-46
Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells.
Wu YF, Cao MF, Gao YP, Chen F, Wang T, Zumbika EP, Qian KX
World journal of gastroenterology 2004 Oct 15;10(20):2944-8
World journal of gastroenterology 2004 Oct 15;10(20):2944-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (M00334-7). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates; Lane 2: human SW579 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Caspase-3 antigen affinity purified monoclonal antibody (Catalog # M00334-7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 20KD. The expected band size for Caspase-3 is at 32KD.
- Additional image