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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- OAAB02398 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- CASP3(Asp175) Antibody Affinity Purified Rabbit Polyclonal antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the CASP3 gene.
- Sample type
- lysatestransiently transfected with the CASP3 gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3 (Asp175) Antibody in CEM cell lysates (35ug/lane). CASP3 (arrow) was detected using the purified Pab.
- Sample type
- CEM cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- CASP3(Asp175) Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CASP3(Asp175) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human brain tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
