- MA5-11516 - Invitrogen Antibodies CASP3 antibody

Submitted references Human Islet Microtissues as an In Vitro and an In Vivo Model System for Diabetes.

Mir-Coll J, Moede T, Paschen M, Neelakandhan A, Valladolid-Acebes I, Leibiger B, Biernath A, Ämmälä C, Leibiger IB, Yesildag B, Berggren PO
International journal of molecular sciences 2021 Feb 11;22(4)

Therapeutic Potentials of Selected Antihypertensive Agents and Their Fixed-Dose Combinations Against Trastuzumab-Mediated Cardiotoxicity.

Olorundare OE, Adeneye AA, Akinsola AO, Ajayi AM, Agede OA, Soyemi SS, Mgbehoma AI, Okoye II, Albrecht RM, Ntambi JM, Crooks PA
Frontiers in pharmacology 2020;11:610331

The protective effects of carnosine in alcohol-induced hepatic injury in rats.

Baykara B, Micili SC, Tugyan K, Tekmen I, Bagriyanik H, Sonmez U, Sonmez A, Oktay G, Yener N, Ozbal S
Toxicology and industrial health 2014 Feb;30(1):25-32

Survival, integration, and differentiation of unrestricted somatic stem cells in the heart.

Ding Z, Burghoff S, Buchheiser A, Kögler G, Schrader J
Cell transplantation 2013;22(1):15-27

Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage.

Vose LR, Vinukonda G, Jo S, Miry O, Diamond D, Korumilli R, Arshad A, Zia MT, Hu F, Kayton RJ, La Gamma EF, Bansal R, Bianco AC, Ballabh P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Oct 30;33(44):17232-46

Erythropoietin prevents lymphoid apoptosis but has no effect on survival in experimental sepsis.

Köroğlu TF, Yılmaz O, Gökmen N, Tuğyan K, Baskın H, Eğrilmez MY
Pediatric research 2013 Aug;74(2):148-53

Captopril and telmisartan treatments attenuate cadmium-induced testicular toxicity in rats.

Fouad AA, Jresat I
Fundamental & clinical pharmacology 2013 Apr;27(2):152-60

Notch signaling change in pulmonary vascular remodeling in rats with pulmonary hypertension and its implication for therapeutic intervention.

Qiao L, Xie L, Shi K, Zhou T, Hua Y, Liu H
PloS one 2012;7(12):e51514

Baicalin induces apoptosis in leukemia HL-60/ADR cells via possible down-regulation of the PI3K/Akt signaling pathway.

Zheng J, Hu JD, Chen YY, Chen BY, Huang Y, Zheng ZH, Liu TB
Asian Pacific journal of cancer prevention : APJCP 2012;13(4):1119-24

Clinicopathological significance of cathepsin D expression in non-small cell lung cancer is conditional on apoptosis-associated protein phenotype: an immunohistochemistry study.

Fan C, Lin X, Wang E
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2012 Aug;33(4):1045-52

Bioengineering functional copolymers. XXI. Synthesis of a novel end carboxyl-trithiocarbonate functionalized poly(maleic anhydride) and its interaction with cancer cells.

Rzayev ZM, Tűrk M, Söylemez EA
Bioorganic & medicinal chemistry 2012 Aug 15;20(16):5053-61

Clinical and prognostic significance of apoptotic profile in patients with newly diagnosed nodal diffuse large B-cell lymphoma (DLBCL).

Markovic O, Marisavljevic D, Cemerikic V, Perunicic M, Savic S, Filipovic B, Mihaljevic B
European journal of haematology 2011 Mar;86(3):246-55

Bioengineering functional copolymers. XIV. Synthesis and interaction of poly(N-isopropylacrylamide-co-3,4-dihydro-2H-pyran-alt-maleic anhydride)s with SCLC cancer cells.

Türk M, Rzayev ZM, Khalilova SA
Bioorganic & medicinal chemistry 2010 Nov 15;18(22):7975-84

Cadmium induces apoptosis in the pituitary gland of Podarcis sicula.

Ferrandino I, Favorito R, Annunziata M, Grimaldi MC
Annals of the New York Academy of Sciences 2009 Apr;1163:386-8

Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.

Sharif M, Whitehouse A, Sharman P, Perry M, Adams M
Arthritis and rheumatism 2004 Feb;50(2):507-15

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Caspase 3 using Caspase 3 Monoclonal Antibody (Product # MA5-11516) on Jurkat Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated for overnight with 1 uM of Etoposide (Lane 2), Jurkat (lane 3) and Jurkat treated for overnight with 3 uM of Staurosporine (lane 4). The blots were probed with Anti-Caspase 3 Mouse Monoclonal Antibody (Product # MA5-11516, 1:25-1:50 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 32 kDa band corresponding to Caspase 3 was observed along with a 17 kDa band in treated lysates corresponding to cleaved Caspase 3. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with Caspase 3 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of lymphoid cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Caspase 3 was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Caspase 3 Mouse Monoclonal Antibody (MA511516, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Caspase 3 using Caspase 3 Monoclonal Antibody (Product # MA5-11516) on denatured Human Jurkat Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Caspase 3 using Caspase 3 Monoclonal Antibody (Product # MA5-11516) on Native Human Jurkat Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 4 Representative photomicrographs of immunohistochemical expression of caspase-3 in cardiac tissue of rats (magnification x400). (A) Normal control, (B) valsartan 10 mg/kg/day, (C) amlodipine 0.25 mg/kg/day, (D) lisinopril 0.035 mg/kg/day, (E) trastuzumab ( TZM ) 2.25 mg/kg/day, (F) TZM + valsartan 5 mg/kg/day, (G) TZM + amlodipine 0.25 mg/kg/day, (H) TZM + lisinopril 0.035 mg/kg, (I) TZM + amlodipine + lisinopril, (J) TZM + valsartan + lisinopril, and (K) intensity score of caspase-3 expression, mean +- SEM ( n = 3), and significant difference denoted by # p < 0.05 vs. normal control or * p < 0.05 vs. TZM by one-way ANOVA followed by Turkey''s post hoc test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure A2 Positive cells in the total, PDX-1 positive and PDX-1 negative populations at the end of 7 days of incubation ( n = 3 donors with n = 6 islet microtissues per donor); ( b ) Total number of cells analyzed represented as DAPI positive nuclei count. ( c ) Apoptosis expressed as the percentage of cleaved caspase-3 positive cells in the total, PDX-1 positive and PDX-1 negative populations at the end of 7 days of incubation ( n = 3 donors with n = 6 islet microtissues per donor); ( d ) Caspase 3/7 activity was analyzed with a lytic assay at the end of 7 days of incubation ( n = 3 donors with n = 6 islet microtissues per donor). Outliers were detected with ROUT's test (Q = 5%). Statistical analysis was performed with Student's t -test.

MA5-11516

Antibody data