NBP1-90125

antibody from Novus Biologicals

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-90125 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-90125, RRID:AB_11040670
Product name: Rabbit Polyclonal Caspase-3 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified. Specificity of human Caspase-3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

CASP3 protein structure - NBP1-90125 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP1-90125] - HUVEC cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with CD31/PECAM-1 Antibody at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP1-90125] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP1-90125] - Staining of human appendix shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
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Experimental details: Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP1-90125] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Caspase-3 Antibody [NBP1-90125] - Analysis in human lymph node and skeletal muscle tissues. Corresponding CASP3 RNA-seq data are presented for the same tissues.