- MA1-16843 - Invitrogen Antibodies CASP3 antibody

Submitted references Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Long X, Wu H, Zhou Y, Wan Y, Kan X, Gong J, Zhao X
Frontiers in nutrition 2022;9:852012

Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats.

Ye YC, Chang ZH, Wang P, Wang YW, Liang J, Chen C, Wang JJ, Sun HT, Wang Y, Li XH
Stem cell research & therapy 2022 Jul 28;13(1):378

Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling.

Xie S, Zhao C, Chen W, Li G, Xiong Z, Tang X, Zhang F, Xiao H
International journal of biological sciences 2021;17(9):2367-2379

Limonoids from Guarea guidonia and Cedrela odorata: Heat Shock Protein 90 (Hsp90) Modulator Properties of Chisomicine D.

Bellone ML, Muñoz Camero C, Chini MG, Dal Piaz F, Hernandez V, Bifulco G, De Tommasi N, Braca A
Journal of natural products 2021 Mar 26;84(3):724-737

Therapeutic effects of syringaldehyde on spinal cord ischemia in rabbits.

Malçok ÜA, Aras AB, Şehitoğlu MH, Akman T, Yüksel Y
Saudi medical journal 2020 Apr;41(4):341-350

Caspase 3 expression and plasma level of Fas ligand as apoptosis biomarkers in inflammatory endotoxemic lung injury.

Fodor RŞ, Georgescu AM, Grigorescu BL, Cioc AD, Veres M, Cotoi OS, Fodor P, Copotoiu SM, Azamfirei L
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie 2016;57(3):951-957

The dual anti-inflammatory and antioxidant activities of natural honey promote cell proliferation and neural regeneration in a rat model of colitis.

Nooh HZ, Nour-Eldien NM
Acta histochemica 2016 Jul;118(6):588-595

Single-cell microinjection assay indicates that 7-hydroxycoumarin induces rapid activation of caspase-3 in A549 cancer cells.

Soto-Nuñez M, Díaz-Morales KA, Cuautle-Rodríguez P, Torres-Flores V, López-González JS, Mandoki-Weitzner JJ, Molina-Guarneros JA
Experimental and therapeutic medicine 2015 Nov;10(5):1789-1795

Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera).

Higes M, Juarranz Á, Dias-Almeida J, Lucena S, Botías C, Meana A, García-Palencia P, Martín-Hernández R
Environmental microbiology reports 2013 Aug;5(4):530-6

Exendin-4 improves hepatocyte injury by decreasing proliferation through blocking NGF/TrkA in diabetic mice.

Gezginci-Oktayoglu S, Sacan O, Yanardag R, Karatug A, Bolkent S
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Effect of vitamin C in reducing the toxicity of endosulfan in liver in rabbits.

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Antiapoptotic effect of angiotensin-II type-1 receptor blockade in renal tubular cells of hyperoxaluric rats.

Tunçdemir M, Demirkesen O, Oztürk M, Atukeren P, Gümüştaş MK, Turan T
Urological research 2010 Apr;38(2):71-80

Expression of caspase-3-dependent apoptosis in mural thrombi leukocytes.

Chu PH, Jung SM, Yeh CH, Wu HH, Shiu TF, She HC, Tseng NM
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2008 Nov;116(11):995-9

Effects of Z-FA.FMK on D-galactosamine/tumor necrosis factor-alpha-induced kidney injury and oxidative stress in mice : effects of Z-FA.FMK on TNF-alpha-mediated kidney injury.

Gezginci-Oktayoglu S, Tunali S, Yanardag R, Bolkent S
Molecular and cellular biochemistry 2008 Feb;309(1-2):9-20

Decreased proliferation precedes growth factor changes after physeal irradiation.

Damron TA, Horton JA, Naqvi A, Margulies B, Strauss J, Grant W, Farnum CE, Spadaro JA
Clinical orthopaedics and related research 2004 May;(422):233-42

Decreased proliferation precedes growth factor changes after physeal irradiation.

Damron TA, Horton JA, Naqvi A, Margulies B, Strauss J, Grant W, Farnum CE, Spadaro JA
Clinical orthopaedics and related research 2004 May;(422):233-42

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of Caspase (19 and 35 kDa) from HEK293 cell extract using Product # MA1-16843. Lanes 1 and 2 contain inactive and active Caspase, respectively.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1) and Jurkat treated with Staurosporine (1uM for 3 hours) (Lane 2). The blot was probed with Anti-Caspase 3 Monoclonal Antibody (CPP32 4-4-18) (Product # MA1-16843, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to Caspase 3 and a 17 kDa corresponding to Cleaved Caspase 3 was observed in the cell line tested. Caspase 3 was observed to reduce upon treatment whereas Cleaved Caspase 3 was enhanced.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Caspase 3 in HEK293 cell extract. Samples were incubated in Caspase 3 monoclonal antibody (Product # MA1-16843). Lanes 1: inactive Caspase; Lane 2: active Caspase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Caspase 3 in 0.5 mg/mL Hek293 lysate. Samples were incubated in Caspase 3 monoclonal (Product # MA1-16843). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockout of Caspase 3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR797315_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Caspase 3 was performed by loading 30 µg of HeLa wild type (Lane 1), HeLa CAS9 (Lane 2) and HeLa Caspase-3 KO (Lane 3) whole cell extracts. The samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Caspase 3 Monoclonal Antibody (CPP32 4-1-18) (Product # MA1-16843) using 1:1,000 dilution and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Caspase 3. Uncharacterized bands were observed at ~70 kDa in all the samples and ~15 kDa in HeLa Caspase-3 KO sample.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Caspase 3 in immersion fixed paraffin-embedded sections of human bladder. Samples were incubated in Caspase 3 monoclonal antibody (Product # MA1-16843) using a dilution of 1:300 for 1 hour at room temperature followed by the anti-mouse IgG VisUCyte HRP polymer. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Caspase 3 in formalin-fixed paraffin-embedded human spleen section. Samples were incubated in Caspase 3 monoclonal antibody (Product # MA1-16843) using a dilution of 1:200 followed by HRP conjugated anti-mouse secondary antibody and DAB reagent. This Caspase 3 antibody generated a specific staining in the cytoplasm of various spleenocytes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Caspase 3 in Rat epithelial cells of the tongue base. Samples were incubated in Caspase 3 monoclonal antibody (Product # MA1-16843). Antigen retrieval method: Citrate buffer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2. Representative western blots showing changes in the protein levels of A549 cell lysates following 7-HC treatment. The A549 cells (1.5x10 6 /ml) were treated with 0.3, 0.6, 0.9 and 1.85 mM 7-HC or ethanol for 24 h. Lysates were prepared and determined as described in Materials and methods. Representative western blots for procaspase-3 and caspase-3 (A) for cells treated with different concentrations of 7-HC for 24 h and (B) following exposure of the cells to 1.85 mM 7-HC for various treatment times. (C) PARP degradation was observed after 24 h of exposure to 1.85 mM 7-HC. PARP, poly (ADP-ribose) polymerase; 7-HC, 7-hydroxycoumarin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Flow cytometry experiment using the annexin V-FITC/PI protocol. (A) U937 cells treated with vehicle (DMSO) for 48 h. (B) U937 cells treated with 20 muM 1 . (C) Western blot analysis of pro-caspase 3 and caspase 3 in cells treated with vehicle (DMSO) and 1 (10 and 20 muM).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Representative images of caspase-3 immunostaining of spinal cords (x200, scale bar=50 um). A) In the control group, few neurons revealed caspase-3-positive staining. B) In the spinal cord ischemia/reperfusion (SCI/R) group, increased number of caspase-3 positivity was observed in the motor neurons. C) Caspase-3 positivity was visible in some neurons in the syringaldehyde-treated group after ischemia, but the number of caspase-3-positive cells was lower than that in SCI/R the group. Arrows refer to caspase-3-positive staining in the motor neurons in the gray matter.

MA1-16843

Antibody data