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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-16332 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Caspase 3 Polyclonal Antibody, Biotin
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- PA5-16341 targets Heat Shock Protein 90 alpha in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and western blot applications and shows reactivity with Human samples. The PA5-16341 immunogen is a synthetic 11-mer peptide, corresponding to aa 2-12 (PEETQTQDQPM-Cys) from the N-terminus end of mouse heat shock protein 90alpha (hsp90alpha). This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Rabbit
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/ml
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with Caspase 3 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of lymphoid cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Caspase 3 using Caspase 3 Polyclonal Antibody (Product # PA5-16332) on denatured Human Jurkat Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Caspase 3 using Caspase 3 Polyclonal Antibody (Product # PA5-16332) on Native Human Jurkat Cells.
- Conjugate
- Biotin
