Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BML-SA320-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA320-0100, RRID:AB_2050937
- Product name
- Caspase-3 (human) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot Analysis of Caspase 3 (human), pAb (BML-SA320): Lane 1: MW Marker, Lane 2: Jurkat Cell Lysate, Lane 3: Jurkat + Staurosporine Cell Lysate, Lane 4: MCF-7 Cell Lysate
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of whole cell extracts from uninduced Jurkat cells (-) and Jurkat cells induced to BS19undergo apoptosis (+) by treatment with human, recombinant soluble TRAIL [SE-721]. One hundred thousand cells were used per lane. The nitrocellulose membrane was probed with Anti-Caspase-3 [SA-320] at 1/5000. Secondary antibody was GAR-AP (1/2000) and the nitrocellulose was developed with BCIP/NBT. P and L indicate positions of the proenzyme and large subunit of the active enzyme, respectively.
