Antibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Immunocytochemistry [5]
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- Product number
- ALX-804-305-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-305-C100, RRID:AB_2050938
- Product name
- Caspase-3 monoclonal antibody (31A1067)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31A1067
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL.
Interleukin-24 inhibits influenza A virus replication in vitro through induction of toll-like receptor 3 dependent apoptosis.
2'-hydroxy C16-ceramide induces apoptosis-associated proteomic changes in C6 glioma cells.
Inhibition of vacuolar ATPase attenuates the TRAIL-induced activation of caspase-8 and modulates the trafficking of TRAIL receptosomes.
Multiple effects of the Na(+)/H (+) antiporter inhibitor HMA on cancer cells.
Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles.
A possible cross-talk between autophagy and apoptosis in generating an immune response in melanoma.
Chondroitin sulphate enhances the antitumor activity of gemcitabine and mitomycin-C in bladder cancer cells with different mechanisms.
RIP3 expression induces a death profile change in U2OS osteosarcoma cells after 5-ALA-PDT.
Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.
NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy.
Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts.
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Protein expression profiling and molecular classification of gastric cancer by the tissue array method.
Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence.
Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma.
Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells.
Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles.
Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells.
Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells.
Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation.
Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3.
Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species.
Reversal of Taxol resistance in hepatoma by cyclosporin A: involvement of the PI-3 kinase-AKT 1 pathway.
Identification of a novel synthetic triterpenoid, methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate, that potently induces caspase-mediated apoptosis in human lung cancer cells.
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat.
The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells.
The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change.
Identification of retinamides that are more potent than N-(4-hydroxyphenyl)retinamide in inhibiting growth and inducing apoptosis of human head and neck and lung cancer cells.
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Sun J, Luo H, Nie W, Xu X, Miao X, Huang F, Wu H, Jin X
International journal of clinical and experimental pathology 2015;8(6):6519-25
International journal of clinical and experimental pathology 2015;8(6):6519-25
Interleukin-24 inhibits influenza A virus replication in vitro through induction of toll-like receptor 3 dependent apoptosis.
Weiss R, Laengle J, Sachet M, Shurygina AP, Kiselev O, Egorov A, Bergmann M
Antiviral research 2015 Nov;123:93-104
Antiviral research 2015 Nov;123:93-104
2'-hydroxy C16-ceramide induces apoptosis-associated proteomic changes in C6 glioma cells.
Kota V, Dhople VM, Fullbright G, Smythe NM, Szulc ZM, Bielawska A, Hama H
Journal of proteome research 2013 Oct 4;12(10):4366-75
Journal of proteome research 2013 Oct 4;12(10):4366-75
Inhibition of vacuolar ATPase attenuates the TRAIL-induced activation of caspase-8 and modulates the trafficking of TRAIL receptosomes.
Horova V, Hradilova N, Jelinkova I, Koc M, Svadlenka J, Brazina J, Klima M, Slavik J, Hyrslova Vaculova A, Andera L
The FEBS journal 2013 Jul;280(14):3436-50
The FEBS journal 2013 Jul;280(14):3436-50
Multiple effects of the Na(+)/H (+) antiporter inhibitor HMA on cancer cells.
Aredia F, Giansanti V, Mazzini G, Savio M, Ortiz LM, Jaadane I, Zaffaroni N, Forlino A, Torriglia A, Scovassi AI
Apoptosis : an international journal on programmed cell death 2013 Dec;18(12):1586-98
Apoptosis : an international journal on programmed cell death 2013 Dec;18(12):1586-98
Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles.
Böing AN, Stap J, Hau CM, Afink GB, Ris-Stalpers C, Reits EA, Sturk A, van Noorden CJ, Nieuwland R
Biochimica et biophysica acta 2013 Aug;1833(8):1844-52
Biochimica et biophysica acta 2013 Aug;1833(8):1844-52
A possible cross-talk between autophagy and apoptosis in generating an immune response in melanoma.
Hossain A, Radwan FF, Doonan BP, God JM, Zhang L, Bell PD, Haque A
Apoptosis : an international journal on programmed cell death 2012 Oct;17(10):1066-78
Apoptosis : an international journal on programmed cell death 2012 Oct;17(10):1066-78
Chondroitin sulphate enhances the antitumor activity of gemcitabine and mitomycin-C in bladder cancer cells with different mechanisms.
Ferro M, Giuberti G, Zappavigna S, Perdonà S, Facchini G, Sperlongano P, Porto S, Di Lorenzo G, Buonerba C, Abbruzzese A, Altieri V, Caraglia M
Oncology reports 2012 Feb;27(2):409-15
Oncology reports 2012 Feb;27(2):409-15
RIP3 expression induces a death profile change in U2OS osteosarcoma cells after 5-ALA-PDT.
Coupienne I, Fettweis G, Piette J
Lasers in surgery and medicine 2011 Sep;43(7):557-64
Lasers in surgery and medicine 2011 Sep;43(7):557-64
Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.
Xu D, Meyer F, Ehlers E, Blasnitz L, Zhang L
The Journal of biological chemistry 2011 May 20;286(20):18261-7
The Journal of biological chemistry 2011 May 20;286(20):18261-7
NF-kappaB inhibition improves the sensitivity of human glioblastoma cells to 5-aminolevulinic acid-based photodynamic therapy.
Coupienne I, Bontems S, Dewaele M, Rubio N, Habraken Y, Fulda S, Agostinis P, Piette J
Biochemical pharmacology 2011 Mar 1;81(5):606-16
Biochemical pharmacology 2011 Mar 1;81(5):606-16
Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcripts.
Fernandez K, Serinagaoglu Y, Hammond S, Martin LT, Martin PT
The American journal of pathology 2010 Jan;176(1):416-34
The American journal of pathology 2010 Jan;176(1):416-34
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP.
Goval JJ, Thielen C, Bourguignon C, Greimers R, Dejardin E, Choi YS, Boniver J, de Leval L
Haematologica 2008 Aug;93(8):1169-77
Haematologica 2008 Aug;93(8):1169-77
Protein expression profiling and molecular classification of gastric cancer by the tissue array method.
Lee HS, Cho SB, Lee HE, Kim MA, Kim JH, Park do J, Kim JH, Yang HK, Lee BL, Kim WH
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 15;13(14):4154-63
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Jul 15;13(14):4154-63
Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence.
Douarre C, Gomez D, Morjani H, Zahm JM, O'donohue MF, Eddabra L, Mailliet P, Riou JF, Trentesaux C
Nucleic acids research 2005;33(7):2192-203
Nucleic acids research 2005;33(7):2192-203
Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma.
Chiang PC, Chien CL, Pan SL, Chen WP, Teng CM, Shen YC, Guh JH
Journal of hepatology 2005 Oct;43(4):679-86
Journal of hepatology 2005 Oct;43(4):679-86
Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells.
Lee JC, Lee CH, Su CL, Huang CW, Liu HS, Lin CN, Won SJ
Carcinogenesis 2005 Oct;26(10):1716-30
Carcinogenesis 2005 Oct;26(10):1716-30
Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles.
Jian W, Arora JS, Oe T, Shuvaev VV, Blair IA
Free radical biology & medicine 2005 Nov 1;39(9):1162-76
Free radical biology & medicine 2005 Nov 1;39(9):1162-76
Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells.
Jin F, Liu X, Zhou Z, Yue P, Lotan R, Khuri FR, Chung LW, Sun SY
Cancer research 2005 Jul 15;65(14):6354-63
Cancer research 2005 Jul 15;65(14):6354-63
Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells.
Tang CH, Hu CC, Wei CW, Wang JJ
FEBS letters 2005 Jan 3;579(1):265-70
FEBS letters 2005 Jan 3;579(1):265-70
Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation.
Jarskog LF, Selinger ES, Lieberman JA, Gilmore JH
The American journal of psychiatry 2004 Jan;161(1):109-15
The American journal of psychiatry 2004 Jan;161(1):109-15
Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3.
Wu YH, Shih SF, Lin JY
The Journal of biological chemistry 2004 Apr 30;279(18):19264-75
The Journal of biological chemistry 2004 Apr 30;279(18):19264-75
Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species.
Ko CH, Shen SC, Chen YC
Free radical biology & medicine 2004 Apr 1;36(7):897-910
Free radical biology & medicine 2004 Apr 1;36(7):897-910
Reversal of Taxol resistance in hepatoma by cyclosporin A: involvement of the PI-3 kinase-AKT 1 pathway.
Lin HL, Lui WY, Liu TY, Chi CW
British journal of cancer 2003 Mar 24;88(6):973-80
British journal of cancer 2003 Mar 24;88(6):973-80
Identification of a novel synthetic triterpenoid, methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate, that potently induces caspase-mediated apoptosis in human lung cancer cells.
Kim KB, Lotan R, Yue P, Sporn MB, Suh N, Gribble GW, Honda T, Wu GS, Hong WK, Sun SY
Molecular cancer therapeutics 2002 Jan;1(3):177-84
Molecular cancer therapeutics 2002 Jan;1(3):177-84
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Chen YC, Shen SC, Lee WR, Hsu FL, Lin HY, Ko CH, Tseng SW
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat.
Zhang WR, Sato K, Iwai M, Nagano I, Manabe Y, Abe K
Brain research 2002 Aug 23;947(1):140-5
Brain research 2002 Aug 23;947(1):140-5
The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells.
Sun SY, Yue P, Chen X, Hong WK, Lotan R
Cancer research 2002 Apr 15;62(8):2430-6
Cancer research 2002 Apr 15;62(8):2430-6
The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change.
Yamaguchi H, Wang HG
Oncogene 2001 Nov 22;20(53):7779-86
Oncogene 2001 Nov 22;20(53):7779-86
Identification of retinamides that are more potent than N-(4-hydroxyphenyl)retinamide in inhibiting growth and inducing apoptosis of human head and neck and lung cancer cells.
Sun SY, Yue P, Kelloff GJ, Steele VE, Lippman SM, Hong WK, Lotan R
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2001 Jun;10(6):595-601
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2001 Jun;10(6):595-601
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Cuddeback SM, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, Singh S, Wang HG
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis for detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. Caspase-3 activation is determined by cleavage of procaspase-3, which generates 17 and 12kDa, larger and smaller catalytic subunit, respectively.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in multiple human tissues using MAb to Caspase-3 (31A1067) (Prod. No. ALX-804-305) at 2µg/ml.Ê Tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in A) RAW and B) NIH 3T3 using MAb to Caspase-3 (31A1067) (Prod. No. ALX-804-305) 2µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with Caspase-3, mAb (31A1067) at 4µg/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of caspase-3 in formalin-fixed, paraffin-embedded human stomach tissue using using MAb to Caspase-3 (31A1067) (Prod. No. ALX-804-305) at 5 ug/ml. High (top panel) and low magnificaton (bottom panel). Caspase-3 staining is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active caspase 3. In most cell types and model systems, cells with active caspase-3 are undergoing apoptosis.