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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- SM7012P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM7012P, RRID:AB_973816
- Product name
- anti Caspase-3 (active + pro)
- Antibody type
- Monoclonal
- Antigen
- Full-length Human Caspase-3 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31A1067
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in HeLa cells. Cells were treated with 2 mM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. Pro Caspase-3 is detected at ~32 kDa. Active/cleaved Caspase-3 (large subunit) is detected at ~14-21 kDa as one or more bands.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in multiple human tissues using SM7012P at 5 µg/ml. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in Human, Mouse and Rat heart lysate, respectively.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Caspase-3 in formalin-fixed, paraffin-embedded human stomach tissue using SM7012P at 5 µg/ml. High (Left panel) and low magnificaton (Right panel). Caspase-3 staining is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active Caspase 3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with Caspase-3 antibody at 4 µg/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis. Cancer/normal adjacent tissue array was used for this test.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
