MAB707

antibody from R&D Systems

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB707

Provider

R&D Systems

Product name

Human Caspase-3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human precursor Caspase-3 and the 18 kDa subunit generated during apoptosis.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P42574


Isotype

IgG

Antibody clone number

84803

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Preeclampsia is associated with alterations in the p53-pathway in villous trophoblast.

Sharp AN, Heazell AE, Baczyk D, Dunk CE, Lacey HA, Jones CJ, Perkins JE, Kingdom JC, Baker PN, Crocker IP
PloS one 2014;9(1):e87621

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PMA synergistically enhances apicularen A-induced cytotoxicity by disrupting microtubule networks in HeLa cells.

Seo KS, Kim JS, Park JH, Song KS, Yun EJ, Park JI, Kweon GR, Yoon WH, Lim K, Hwang BD
BMC cancer 2014 Jan 22;14:36

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Interactions between important regulatory proteins and human alphaB crystallin.

Ghosh JG, Shenoy AK Jr, Clark JI
Biochemistry 2007 May 29;46(21):6308-17

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Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.

Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L142-50

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Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans.

Vallon M, Essler M
The Journal of biological chemistry 2006 Nov 10;281(45):34179-88

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Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.

You RI, Chen MC, Wang HW, Chou YC, Lin CH, Hsieh SL
Cancer research 2006 Mar 15;66(6):3051-61

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Precursor Caspase-3 and p18 Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase-3 Monoclonal Antibody (Catalog # MAB707), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for precursor Caspase-3 and the p18 subunit at approximately 36 and 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human Caspase-3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caspase-3 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase-3 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.