NBP2-16369

antibody from Novus Biologicals

Targeting: PDIA3 ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16369

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ERp57/PDIA3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - U87 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - Mouse testis. ERp57 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.