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LearnAMAb90988
antibody from Atlas Antibodies
Targeting: PDIA3
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AMAb90988 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90988, RRID:AB_2665750
- Product name
- Anti-PDIA3
- Antibody type
- Monoclonal
- Description
- Monoclonal Antibody against Human PDIA3, Clone ID: CL2444, Gene description: protein disulfide isomerase family A, member 3, Alternative Gene Names: ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, Validated applications: ICC, IHC, WB, Uniprot ID: G5EA52, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CL2444
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Lysosomal membrane proteins LAMP1 and LIMP2 are segregated in the Golgi apparatus independently of their clathrin adaptor binding motif
Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
NRF3 suppresses squamous carcinogenesis, involving the unfolded protein response regulator HSPA5
Prenylcysteine oxidase 1, an emerging player in atherosclerosis
BioID reveals an ATG9A interaction with ATG13‐ATG101 in the degradation of p62/SQSTM1‐ubiquitin clusters
IRE1α Promotes Zika Virus Infection via XBP1
Salinomycin kills cancer stem cells by sequestering iron in lysosomes
Ecard J, Lian Y, Divoux S, Gouveia Z, Vigne E, Perez F, Boncompain G, Marks M
Molecular Biology of the Cell 2024;35(3)
Molecular Biology of the Cell 2024;35(3)
Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling
Kalarikkal M, Saikia R, Oliveira L, Bhorkar Y, Lonare A, Varshney P, Dhamale P, Majumdar A, Joseph J
EMBO Reports 2024;25(10):4226-4251
EMBO Reports 2024;25(10):4226-4251
Rodriguez R, Cañeque T, Baron L, Müller S, Carmona A, Colombeau L, Versini A, Sabatier M, Sampaio J, Mishima E, Picard-Bernes A, Solier S, Zheng J, Proneth B, Thoidingjam L, Gaillet C, Grimaud L, Fraser C, Szylo K, Bonnet C, Charafe E, Ginestier C, Santofimia P, Dusetti N, Iovanna J, Cunha A, Pittau G, Hammel P, Tzanis D, Bonvalot S, Watson S, Stockwell B, Conrad M, Ubellacker J
2024
2024
NRF3 suppresses squamous carcinogenesis, involving the unfolded protein response regulator HSPA5
Gurri S, Siegenthaler B, Cangkrama M, Restivo G, Huber M, Saliba J, Dummer R, Blank V, Hohl D, Werner S
EMBO Molecular Medicine 2023;15(11)
EMBO Molecular Medicine 2023;15(11)
Prenylcysteine oxidase 1, an emerging player in atherosclerosis
Banfi C, Baetta R, Barbieri S, Brioschi M, Guarino A, Ghilardi S, Sandrini L, Eligini S, Polvani G, Bergman O, Eriksson P, Tremoli E
Communications Biology 2021;4(1)
Communications Biology 2021;4(1)
BioID reveals an ATG9A interaction with ATG13‐ATG101 in the degradation of p62/SQSTM1‐ubiquitin clusters
Kannangara A, Poole D, McEwan C, Youngs J, Weerasekara V, Thornock A, Lazaro M, Balasooriya E, Oh L, Soderblom E, Lee J, Simmons D, Andersen J
EMBO reports 2021;22(10)
EMBO reports 2021;22(10)
IRE1α Promotes Zika Virus Infection via XBP1
Kolpikova E, Tronco A, Den Hartigh A, Jackson K, Iwawaki T, Fink S
Viruses 2020;12(3):278
Viruses 2020;12(3):278
Salinomycin kills cancer stem cells by sequestering iron in lysosomes
Mai T, Hamaï A, Hienzsch A, Cañeque T, Müller S, Wicinski J, Cabaud O, Leroy C, David A, Acevedo V, Ryo A, Ginestier C, Birnbaum D, Charafe-Jauffret E, Codogno P, Mehrpour M, Rodriguez R
Nature Chemistry 2017;9(10):1025-1033
Nature Chemistry 2017;9(10):1025-1033
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using AMAb90988 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
