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LearnAF8219
antibody from R&D Systems
Targeting: PDIA3
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF8219 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ERp57/PDIA3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ERp57/PDIA3 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P30101- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice.
Larsson S, Voss U
BMC gastroenterology 2018 Nov 21;18(1):175
BMC gastroenterology 2018 Nov 21;18(1):175
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse ERp57/PDIA3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ERp57/PDIA3 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ERp57/PDIA3 in HeLa Human Cell Line. ERp57/PDIA3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ERp57/PDIA3 in Human Liver. ERp57/PDIA3 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human ERp57/PDIA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8219) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
