PAB29388

antibody from Abnova Corporation

Targeting: PDIA3 ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29388

Provider

Abnova Corporation

Product name

PDIA3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human PDIA3.

Antigen sequence

PTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPA
SVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSE
FLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIIL
FRPSHLTNKFEDK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with PDIA3 polyclonal antibody (Cat # PAB29388) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with PDIA3 polyclonal antibody (Cat # PAB29388) at 1-4 ug/mL concentration shows positivity in endoplasmic reticulum.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with PDIA3 polyclonal antibody (Cat # PAB29388) shows cytoplasmic positivity in glandular cells at 1:2500-1:5000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)