HPA002645

PDIA3 antibody from Atlas Antibodies

ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Validated within the Antibodypedia Validation Initative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA002645

Antibody Data

Product number

HPA002645

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA002645, RRID:AB_611462

Product name

Anti-PDIA3

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

HMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWR
NRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFG
LESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALE
RFLQDYFDGNLKRYLKSEPIPESNDGP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome
Kashyap M, Harsha H, Renuse S, Pawar H, Sahasrabuddhe N, Kim M, Marimuthu A, Keerthikumar S, Muthusamy B, Kandasamy K, Subbannayya Y, Prasad T, Mahmood R, Chaerkady R, Meltzer S, Kumar R, Rustgi A, Pandey A
Cancer Biology & Therapy 2014 October;10(8):796-810

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human liver tissueLane 5: Human tonsil tissue

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

HUMAN

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human thyroid gland shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human thyroid gland and skeletal muscle tissues using Anti-PDIA3 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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