MA5-21012

antibody from Invitrogen Antibodies

Targeting: CLEC4M CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, LSIGN

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-21012

Provider

Invitrogen Antibodies

Product name

CD299 (DC-SIGN/L) Monoclonal Antibody (2G1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: SQRNWHDSVT ACQEVRAQLV VIKTAEEQNF LQLQTSRSNR FSWMGLSDLN QEGTWQWVDG SPLSPSFQRY WNSGEPNNSG NEDCAEFSGS GWNDNRCDVD NYWICKKPAA

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2G1

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Monitoring Viral Entry in Real-Time Using a Luciferase Recombinant Vesicular Stomatitis Virus Producing SARS-CoV-2, EBOV, LASV, CHIKV, and VSV Glycoproteins.

Lay Mendoza MF, Acciani MD, Levit CN, Santa Maria C, Brindley MA
Viruses 2020 Dec 17;12(12)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 rVSVG/EBOV entry kinetics: ( A ) VeroS cells were infected with rVSVG/EBOV at multiple MOIs, inoculum was removed after 1 h, and monitored for luciferase production over time. ( B ) VeroS, HEK293T, and HAP1 cells were infected with rVSVG/EBOV (MOI 1), inoculum was not removed, and monitored for luciferase production over time. Right panel zooms in to display the signals observed in HAP1 and HEK293T cells. ( C ) Immunoblots demonstrating production of the transfected receptors in HEK293T cells. ( D ) HEK293T cells transfected with the indicated receptors were infected with rVSVG/EBOV and monitored for luciferase production over time. Each experiment was repeated in duplicate, three independent times. Data shown are the averages of averages and corresponding SEM of three independent trials.