LS-B2003

antibody from LSBio

Targeting: KRT19 CK19, K19, K1CS, MGC15366

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B2003

Provider

LSBio

Product name

PathPlus™ KRT19 / CK19 / Cytokeratin 19 Antibody (clone A53-B/A2) LS-B2003

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

A53-B/A2

Storage

Store at 4°C. Store undiluted.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 19 antibody (clone A53-B/A2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor 546 secondary antibody and DAPI (nuclei). Images were acquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor 546 secondary antibody and DAPI (nuclei). Images were acquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor 546 secondary antibody and DAPI (nuclei). Images were acquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.