MA1-91210

antibody from Invitrogen Antibodies

Targeting: KRT19 CK19, K19, K1CS, MGC15366

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-91210

Provider

Invitrogen Antibodies

Product name

Cytokeratin 19 Monoclonal Antibody (BA16)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-91210 detects Cytokeratin 19 from human samples.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BA16

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds.

Hahn JM, Combs KA, Lloyd CM, McFarland KL, Boyce ST, Supp DM
PloS one 2019;14(3):e0213325

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 19 Monoclonal Antibody (BA16) (Product # MA1-91210) and a 44 kDa band corresponding to KRT19 was observed across cell lines tested. An additional band around 38 kDa has been observed which is reported for Cytokeratin 19 expression. Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), HT-29 (Lane 4), COLO 205 (Lane 5) and U-2 OS (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (BA16) (Product # MA1-91210) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.