Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 628508 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#628508, RRID:AB_2566632
- Product name
- Alexa Fluor® 488 anti-Cytokeratin 19
- Antibody type
- Monoclonal
- Antigen
- Human mammary carcinoma cell line MCF-7
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antibody clone number
- A53-B/A2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes at 37°C, permeabilized with 0.5% Triton X-100 buffer for ten minutes at room temperature, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with 5 µg/mL anti-Cytokeratin 19 (clone A53-B/A2) Alexa Fluor® 488 (green). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
- Conjugate
- Green dye