42-9898-82

antibody from Invitrogen Antibodies
Targeting: KRT19 CK19, K19, K1CS, MGC15366
Provider product page for 42-9898-82
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
42-9898-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Cytokeratin 19 Monoclonal Antibody (BA17), eFluor™ 615, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This BA17 monoclonal antibody reacts with human cytokeratin 19 (CK19), a 44-kDa type I (acidic) intermediate filament protein that lacks the non-alpha-helical tail domain present in other keratins. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. Cytokeratin 19 is expressed in a wide variety of simple and stratified epithelial tissue. Moreover, cytokeratin 19 expression can be induced by vitamin A, SV40 transformation, and cancer. A soluble form of cytokeratin 19 generated by caspase 3 cleavage has also been found to be secreted by cancer cells, thus possibly indicating tumor metastasis. Cytokeratin 19 often exists as a heterodimer with cytokeratin 7, a type II keratin. Applications Reported: This BA17 antibody has been reported for use in immunocytochemical and immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue. Applications Tested: This BA17 antibody has been tested by immunocytochemistry (ICC) on fixed and permeabilized MCF7 cells at less than or equal to 10 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
BA17
Vial size
100 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
KRT19 protein structure - 42-9898-82 shown in red.