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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [12]
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- Product number
- CF190116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acidic Cytokeratin Monoclonal Antibody (OTI1D10), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- OTI1D10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with 55 different plasmids of pCMV6-ENTRY with CK (1,2,4,5,6a,6b,6c,7,8,9,12,13,14,15,16,17,18 v1,18 v2,19,20,24,25,26,27,28,31,32,33a,33b,34,35,36,37,38,39,40,71,72 v1,72 v3,73,74,75,76,77,78,79,80 v1,80 v2,81,82,83,84,85,86 and 222) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CK antibody. As shown in the figure, KRT12, 15, 16, 17, 19, 24, 25, 26,27, 28, 37 and 39 were positive, while all the others were negative. (1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human bladder tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)
