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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- MA1-06329 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 19 Monoclonal Antibody (RCK108)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-06329 detects cytokeratin 19 in human and zebrafish samples.
- Antibody clone number
- RCK108
- Concentration
- 1 mg/mL
Submitted references Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.
High expression of Galectin-1 in pancreatic stellate cells plays a role in the development and maintenance of an immunosuppressive microenvironment in pancreatic cancer.
Dormant but migratory tumour cells in desmoplastic stroma of invasive ductal carcinomas.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
Identification of expandable human hepatic progenitors which differentiate into mature hepatic cells in vivo.
Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, Matsubara H, Saito Y, Nakayama Y, Tomonaga T
Scientific reports 2016 Sep 29;6:34472
Scientific reports 2016 Sep 29;6:34472
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
Ouyang H, Xue Y, Lin Y, Zhang X, Xi L, Patel S, Cai H, Luo J, Zhang M, Zhang M, Yang Y, Li G, Li H, Jiang W, Yeh E, Lin J, Pei M, Zhu J, Cao G, Zhang L, Yu B, Chen S, Fu XD, Liu Y, Zhang K
Nature 2014 Jul 17;511(7509):358-61
Nature 2014 Jul 17;511(7509):358-61
A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.
Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, Hoshino I, Nishimori T, Matsubara H, Tomonaga T
Journal of cell science 2013 Oct 15;126(Pt 20):4721-31
Journal of cell science 2013 Oct 15;126(Pt 20):4721-31
High expression of Galectin-1 in pancreatic stellate cells plays a role in the development and maintenance of an immunosuppressive microenvironment in pancreatic cancer.
Tang D, Yuan Z, Xue X, Lu Z, Zhang Y, Wang H, Chen M, An Y, Wei J, Zhu Y, Miao Y, Jiang K
International journal of cancer 2012 May 15;130(10):2337-48
International journal of cancer 2012 May 15;130(10):2337-48
Dormant but migratory tumour cells in desmoplastic stroma of invasive ductal carcinomas.
Raviraj V, Zhang H, Chien HY, Cole L, Thompson EW, Soon L
Clinical & experimental metastasis 2012 Mar;29(3):273-92
Clinical & experimental metastasis 2012 Mar;29(3):273-92
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
Sumitran-Holgersson S, Nowak G, Thowfeequ S, Begum S, Joshi M, Jaksch M, Kjaeldgaard A, Jorns C, Ericzon BG, Tosh D
Cell transplantation 2009;18(2):183-93
Cell transplantation 2009;18(2):183-93
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
Sumitran-Holgersson S, Nowak G, Thowfeequ S, Begum S, Joshi M, Jaksch M, Kjaeldgaard A, Jorns C, Ericzon BG, Tosh D
Cell transplantation 2009;18(2):183-93
Cell transplantation 2009;18(2):183-93
Identification of expandable human hepatic progenitors which differentiate into mature hepatic cells in vivo.
Nowak G, Ericzon BG, Nava S, Jaksch M, Westgren M, Sumitran-Holgersson S
Gut 2005 Jul;54(7):972-9
Gut 2005 Jul;54(7):972-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Cytokeratin 19 Monoclonal Antibody (RCK108) (Product # MA1-06329) and a 44 kDa band corresponding to KRT19 was observed across cell lines tested. An additional band around 38 kDa has been observed which is reported for Cytokeratin 19 expression. Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), SK-BR-3 (Lane 2) and COLO 205 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti- Mouse (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunostaining of cytokeratin 19 on mammal carcinoma tissue using Product # MA1-06329.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (RCK108) (Product # MA1-06329) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 9 days old zebrafish embryo using Cytokeratin 19 monoclonal antibody (Product # MA1-06329).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 9 days old zebrafish embryo using Cytokeratin 19 monoclonal antibody (Product # MA1-06329).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 9 days old zebrafish embryo using Cytokeratin 19 monoclonal antibody (Product # MA1-06329).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on frozen section of human liver bile duct epithelium stained with Cytokeratin 19 monoclonal antibody (Product # MA1-06329).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
