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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MA1-19479 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 19 Monoclonal Antibody (A53-B/A2), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- A53-B/A2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Stage-specific expression of the intermediate filament protein cytokeratin 13 in luminal epithelial cells of secretory phase human endometrium and peri-implantation stage rabbit endometrium.
Stage-specific expression of the intermediate filament protein cytokeratin 13 in luminal epithelial cells of secretory phase human endometrium and peri-implantation stage rabbit endometrium.
Olson GE, Winfrey VP, Blaeuer GL, Palisano JR, NagDas SK
Biology of reproduction 2002 Apr;66(4):1006-15
Biology of reproduction 2002 Apr;66(4):1006-15
Stage-specific expression of the intermediate filament protein cytokeratin 13 in luminal epithelial cells of secretory phase human endometrium and peri-implantation stage rabbit endometrium.
Olson GE, Winfrey VP, Blaeuer GL, Palisano JR, NagDas SK
Biology of reproduction 2002 Apr;66(4):1006-15
Biology of reproduction 2002 Apr;66(4):1006-15
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 (Product # MA1-19479) on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of biotinylated mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 19 was detected at approximately 41kDa.
- Conjugate
- Biotin
