PA5-111755

antibody from Invitrogen Antibodies

Targeting: TRPA1 ANKTM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-111755

Provider

Invitrogen Antibodies

Product name

TRPA1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.8 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TRPA1 was performed in rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates. Lane 1,3,4: TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. Lane 2,5,6: TRPA1 (extracellular) Antibody, preincubated with the control peptide antigen.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TRPA1 was performed in rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates. Lane 1,3,4: TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. Lane 2,5,6: TRPA1 (extracellular) Antibody, preincubated with the control peptide antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TRPA1 in rat PC12 cells. A) Extracellular staining of cells using TRPA1 (extracellular) Antibody (Product # PA5-111755), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B) Live view of the cells. C) Merged image of A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TRPA1 in rat DRG tissue sections using TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. A) TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B) Nuclear staining using DAPI as the counterstain. C) Merged image of A and B.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of TRPA1 was performed in PC-12 lysates. 1) PC-12 lysates. 2) PC-12 lysates, TRPA1 (extracellular) Antibody (Product # PA5-111755) (6.5 µg), and protein A beads. 3) TRPA1 (extracellular) Antibody and protein A beads.