PA5-111755
antibody from Invitrogen Antibodies
Targeting: TRPA1
ANKTM1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-111755 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPA1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRPA1 was performed in rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates. Lane 1,3,4: TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. Lane 2,5,6: TRPA1 (extracellular) Antibody, preincubated with the control peptide antigen.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRPA1 was performed in rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates. Lane 1,3,4: TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. Lane 2,5,6: TRPA1 (extracellular) Antibody, preincubated with the control peptide antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRPA1 in rat PC12 cells. A) Extracellular staining of cells using TRPA1 (extracellular) Antibody (Product # PA5-111755), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B) Live view of the cells. C) Merged image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TRPA1 in rat DRG tissue sections using TRPA1 (extracellular) Antibody (Product # PA5-111755) at a dilution of 1:200. A) TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B) Nuclear staining using DAPI as the counterstain. C) Merged image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRPA1 was performed in PC-12 lysates. 1) PC-12 lysates. 2) PC-12 lysates, TRPA1 (extracellular) Antibody (Product # PA5-111755) (6.5 µg), and protein A beads. 3) TRPA1 (extracellular) Antibody and protein A beads.