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LearnACC-037-25UL
antibody from Invitrogen Antibodies
Targeting: TRPA1
ANKTM1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- ACC-037-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPA1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat DRG (lanes 1,2), non-differentiated PC12cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates: - 1,3,4. Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:200).2,5,6. Anti-TRPA1 (extracellular) Antibody , preincubated with TRPA1 (extracellular) Blocking Peptide (#BLP-CC037).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPA1 in rat PC12 cells - Cell surface detection of TRPA1 in intact living rat PC12 cells. A. Extracellular staining of cells using Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merged image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPA1 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPA1 (extracellular) Antibody (#ACC-037) (1:200). A. TRPA1 staining (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of TRPA1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-TRPA1 (extracellular) Antibody (#ACC-037), (5μg) + goat- Anti-rabbit-FITC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PC-12 lysates: - 1. PC-12 lysates. 2. PC-12 lysates + Anti-TRPA1 (extracellular) Antibody (#ACC-037) (6.5 µg) + protein A beads.3. Anti-TRPA1 (extracellular) Antibody + protein A beads.
