PA1-46159

antibody from Invitrogen Antibodies

Targeting: TRPA1 ANKTM1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-46159

Provider

Invitrogen Antibodies

Product name

TRPA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The target sequence has 69% sequence homology with rat. Suggested positive control: human brain membrane fraction.

Reactivity

Human, Mouse, Rat, Guinea Pig, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Interleukin 1 beta-induced calcium signaling via TRPA1 channels promotes mitogen-activated protein kinase-dependent mesangial cell proliferation.

Soni H, Kumar R, Kanthakumar P, Adebiyi A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jul;35(7):e21729

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Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Cojocaru F, Şelescu T, Domocoş D, Măruţescu L, Chiritoiu G, Chelaru NR, Dima S, Mihăilescu D, Babes A, Cucu D
Scientific reports 2021 Jan 21;11(1):2018

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Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice.

Wen J, Bo T, Zhang X, Wang Z, Wang D
The Journal of experimental biology 2020 Jun 4;223(Pt 11)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of TRPA1 in human brain membrane fraction using 2 µg/mL (Product # PA1-46159).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TRPA1 in mouse intestine. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TRPA1 in A549 cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Expression of TRPA1 in human PDAC cell lines. ( A ) Expression pattern of the TRPA1 protein in PDAC cell lines. Blots from whole-cell lysates (50 µg) obtained from three PDAC cell lines, the human pancreatic ductal cells, wild-type HEK293T and HEK293T/A1-transfected cell lines were probed with antibodies against TRPA1 and Hsp90 on the same gel. Cropped gel is presented here with full gel available in Supplemental Fig. 1 . ( B ) Densitometry quantification of the bands from panel A was performed with Quantity One software. The results are expressed as the ratio of the expression of TRPA1 to Hsp 90. ( C ) Levels of TRPA1 mRNA were determined by real-time RT-PCR, and a comparative Ct method (2 -DeltaDeltaCt ) was used for the relative mRNA quantification. Hu18S was used as endogenous gene. The values in ( B , C ) are shown as the means +- SD; n = 3. * p < 0.05 vs. control.