PAB28598

antibody from Abnova Corporation

Targeting: UBE2T FANCT, HSPC150

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28598

Provider

Abnova Corporation

Product name

UBE2T polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human UBE2T.

Reactivity

Human

Host

Rabbit

Antigen sequence

FKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRI
CLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNP
DDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQ
KADEEEMLDNLPEAGDSRVHNSTQKRK


Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with UBE2T polyclonal antibody (Cat#PAB28598) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with UBE2T polyclonal antibody (Cat#PAB28598) at 4 ug/ml shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with UBE2T polyclonal antibody (Cat#PAB28598) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)