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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- R35368 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- UBE2T Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific UBE2T antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the UBE2T antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of K562 lysate with UBE2T antibody at 0.1ug/ml. Predicted/observed molecular weight: ~22kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of K562 lysate with UBE2T antibody at 0.1ug/ml. Predicted/observed molecular weight: ~22kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A) K562 and B) ThP-1 cell lysate with UBE2T antibody. Predicted molecular weight: ~22 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A) A431 nuclear fraction, B) HEK293 nuclear fraction, C) HeLa nuclear fraction, D) Jurkat nuclear fraction and E) HepG2 nuclear fraction lysate with UBE2T antibody. Predicted molecular weight: ~22 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with UBE2T antibody (green) at 10ug/ml, DAPI nuclear stain (blue) and Phalloidin (red).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with UBE2T antibody (green) at 10ug/ml, DAPI nuclear stain (blue) and Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human HeLa cells with UBE2T antibody (blue) at 10ug/ml and naive goat Ig (black).
