TA501645

antibody from OriGene

Targeting: UBE2T FANCT, HSPC150

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501645

Provider

OriGene

Proper citation

OriGene Cat#TA501645, RRID:AB_11130215

Product name

Anti-UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Antibody type

Monoclonal

Description

Anti-UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Host

Mouse

Conjugate

Unconjugated

Epitope

UBE2T

Isotype

IgG

Antibody clone number

OTI2F5

Vial size

100 µl

Concentration

0.33 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (RC200748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2T monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-UBE2T mouse monoclonal antibody (TA501645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T(RC200748).

Validation comment

IF