Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-60412 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60412, RRID:AB_905315
- Product name
- Rabbit Polyclonal CHD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CHD6 chromatin remodeler is a negative modulator of influenza virus replication that relocates to inactive chromatin upon infection.
Alfonso R, Lutz T, Rodriguez A, Chavez JP, Rodriguez P, Gutierrez S, Nieto A
Cellular microbiology 2011 Dec;13(12):1894-906
Cellular microbiology 2011 Dec;13(12):1894-906
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CHD3 Antibody [NB100-60412] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody NB100-60412 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by previous lot 1 of this antibody and by rabbit anti-CHD3 antibody NB100-60413, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHD3 Antibody [NB100-60412] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB