628002

antibody from BioLegend
Targeting: TPX2 C20orf1, C20orf2, DIL-2, p100
Provider product page for 628002
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
628002 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#628002, RRID:AB_2287863
Product name
Purified anti-TPX2
Antibody type
Monoclonal
Antigen
Amino Acid: 1-220 of human TPX2
Description
The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, α-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Isotype
IgG
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
The antibody solution should be stored undiluted at 4 °C.
TPX2 protein structure - 628002 shown in red.