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- Antigen structure
- References [29]
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB500-179 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-179, RRID:AB_10002747
- Product name
- Rabbit Polyclonal TPX2 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references RanGTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes.
Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit.
The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.
TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells.
P190RhoGAP prevents mitotic spindle fragmentation and is required to activate Aurora A kinase at acentriolar poles.
USP35 regulates mitotic progression by modulating the stability of Aurora B.
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells.
Adducin-1 is essential for spindle pole integrity through its interaction with TPX2.
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics.
FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence.
Bcl2l10, a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytes.
The Clathrin-dependent Spindle Proteome.
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
Enhanced protein degradation by branched ubiquitin chains.
Microtubule-dependent regulation of mitotic protein degradation.
The cytoskeletal protein RHAMM and ERK1/2 activity maintain the pluripotency of murine embryonic stem cells.
NQO1 prevents radiation-induced aneuploidy by interacting with Aurora-A.
Aurora A kinase regulates mammary epithelial cell fate by determining mitotic spindle orientation in a Notch-dependent manner.
Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.
Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes.
Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux.
Identification of a physiological E2 module for the human anaphase-promoting complex.
A survivin-ran complex regulates spindle formation in tumor cells.
Tumor cell dependence on Ran-GTP-directed mitosis.
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
Drutovic D, Duan X, Li R, Kalab P, Solc P
The EMBO journal 2020 Jan 2;39(1):e101689
The EMBO journal 2020 Jan 2;39(1):e101689
Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit.
Naso FD, Sterbini V, Crecca E, Asteriti IA, Russo AD, Giubettini M, Cundari E, Lindon C, Rosa A, Guarguaglini G
Cells 2020 Feb 6;9(2)
Cells 2020 Feb 6;9(2)
The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.
Capalbo L, Bassi ZI, Geymonat M, Todesca S, Copoiu L, Enright AJ, Callaini G, Riparbelli MG, Yu L, Choudhary JS, Ferrero E, Wheatley S, Douglas ME, Mishima M, D'Avino PP
Nature communications 2019 Oct 4;10(1):4513
Nature communications 2019 Oct 4;10(1):4513
TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells.
van Gijn SE, Wierenga E, van den Tempel N, Kok YP, Heijink AM, Spierings DCJ, Foijer F, van Vugt MATM, Fehrmann RSN
Oncogene 2019 Feb;38(6):852-867
Oncogene 2019 Feb;38(6):852-867
P190RhoGAP prevents mitotic spindle fragmentation and is required to activate Aurora A kinase at acentriolar poles.
Manukyan A, Sargsyan L, Parsons SJ, Stukenberg PT
Chromosoma 2018 Sep;127(3):375-386
Chromosoma 2018 Sep;127(3):375-386
USP35 regulates mitotic progression by modulating the stability of Aurora B.
Park J, Kwon MS, Kim EE, Lee H, Song EJ
Nature communications 2018 Feb 15;9(1):688
Nature communications 2018 Feb 15;9(1):688
Distribution of Eg5 and TPX2 in mitosis: Insight from CRISPR tagged cells.
Mann BJ, Wadsworth P
Cytoskeleton (Hoboken, N.J.) 2018 Dec;75(12):508-521
Cytoskeleton (Hoboken, N.J.) 2018 Dec;75(12):508-521
Adducin-1 is essential for spindle pole integrity through its interaction with TPX2.
Hsu WH, Wang WJ, Lin WY, Huang YM, Lai CC, Liao JC, Chen HC
EMBO reports 2018 Aug;19(8)
EMBO reports 2018 Aug;19(8)
HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development.
Connell M, Chen H, Jiang J, Kuan CW, Fotovati A, Chu TL, He Z, Lengyell TC, Li H, Kroll T, Li AM, Goldowitz D, Frappart L, Ploubidou A, Patel MS, Pilarski LM, Simpson EM, Lange PF, Allan DW, Maxwell CA
eLife 2017 Oct 10;6
eLife 2017 Oct 10;6
Regulation of Kif15 localization and motility by the C-terminus of TPX2 and microtubule dynamics.
Mann BJ, Balchand SK, Wadsworth P
Molecular biology of the cell 2017 Jan 1;28(1):65-75
Molecular biology of the cell 2017 Jan 1;28(1):65-75
FSH Regulates mRNA Translation in Mouse Oocytes and Promotes Developmental Competence.
Franciosi F, Manandhar S, Conti M
Endocrinology 2016 Feb;157(2):872-82
Endocrinology 2016 Feb;157(2):872-82
Bcl2l10, a new Tpx2 binding partner, is a master regulator of Aurora kinase A in mouse oocytes.
Lee SY, Kim EY, Kim KH, Lee KA
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3296-3305
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3296-3305
The Clathrin-dependent Spindle Proteome.
Rao SR, Flores-Rodriguez N, Page SL, Wong C, Robinson PJ, Chircop M
Molecular & cellular proteomics : MCP 2016 Aug;15(8):2537-53
Molecular & cellular proteomics : MCP 2016 Aug;15(8):2537-53
A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.
Barr AR, Bakal C
Scientific reports 2015 Jun 3;5:10564
Scientific reports 2015 Jun 3;5:10564
The selective inhibition of protein phosphatase-1 results in mitotic catastrophe and impaired tumor growth.
Winkler C, De Munter S, Van Dessel N, Lesage B, Heroes E, Boens S, Beullens M, Van Eynde A, Bollen M
Journal of cell science 2015 Dec 15;128(24):4526-37
Journal of cell science 2015 Dec 15;128(24):4526-37
Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe.
Cornago M, Garcia-Alberich C, Blasco-Angulo N, Vall-Llaura N, Nager M, Herreros J, Comella JX, Sanchis D, Llovera M
Cell death & disease 2014 Oct 2;5:e1435
Cell death & disease 2014 Oct 2;5:e1435
Enhanced protein degradation by branched ubiquitin chains.
Meyer HJ, Rape M
Cell 2014 May 8;157(4):910-21
Cell 2014 May 8;157(4):910-21
Microtubule-dependent regulation of mitotic protein degradation.
Song L, Craney A, Rape M
Molecular cell 2014 Jan 23;53(2):179-92
Molecular cell 2014 Jan 23;53(2):179-92
The cytoskeletal protein RHAMM and ERK1/2 activity maintain the pluripotency of murine embryonic stem cells.
Jiang J, Mohan P, Maxwell CA
PloS one 2013;8(9):e73548
PloS one 2013;8(9):e73548
NQO1 prevents radiation-induced aneuploidy by interacting with Aurora-A.
Park MT, Oh ET, Song MJ, Lee H, Choi EK, Park HJ
Carcinogenesis 2013 Nov;34(11):2470-85
Carcinogenesis 2013 Nov;34(11):2470-85
Aurora A kinase regulates mammary epithelial cell fate by determining mitotic spindle orientation in a Notch-dependent manner.
Regan JL, Sourisseau T, Soady K, Kendrick H, McCarthy A, Tang C, Brennan K, Linardopoulos S, White DE, Smalley MJ
Cell reports 2013 Jul 11;4(1):110-23
Cell reports 2013 Jul 11;4(1):110-23
Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells.
Hasegawa K, Ryu SJ, Kaláb P
The Journal of cell biology 2013 Jan 21;200(2):151-61
The Journal of cell biology 2013 Jan 21;200(2):151-61
Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes.
Chen J, Torcia S, Xie F, Lin CJ, Cakmak H, Franciosi F, Horner K, Onodera C, Song JS, Cedars MI, Ramalho-Santos M, Conti M
Nature cell biology 2013 Dec;15(12):1415-23
Nature cell biology 2013 Dec;15(12):1415-23
Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.
Roscioli E, Di Francesco L, Bolognesi A, Giubettini M, Orlando S, Harel A, Schininà ME, Lavia P
The Journal of cell biology 2012 Feb 20;196(4):435-50
The Journal of cell biology 2012 Feb 20;196(4):435-50
Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux.
Ma N, Tulu US, Ferenz NP, Fagerstrom C, Wilde A, Wadsworth P
Molecular biology of the cell 2010 Mar 15;21(6):979-88
Molecular biology of the cell 2010 Mar 15;21(6):979-88
Identification of a physiological E2 module for the human anaphase-promoting complex.
Williamson A, Wickliffe KE, Mellone BG, Song L, Karpen GH, Rape M
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18213-8
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18213-8
A survivin-ran complex regulates spindle formation in tumor cells.
Xia F, Canovas PM, Guadagno TM, Altieri DC
Molecular and cellular biology 2008 Sep;28(17):5299-311
Molecular and cellular biology 2008 Sep;28(17):5299-311
Tumor cell dependence on Ran-GTP-directed mitosis.
Xia F, Lee CW, Altieri DC
Cancer research 2008 Mar 15;68(6):1826-33
Cancer research 2008 Mar 15;68(6):1826-33
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
Bird AW, Hyman AA
The Journal of cell biology 2008 Jul 28;182(2):289-300
The Journal of cell biology 2008 Jul 28;182(2):289-300
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: TPX2 Antibody [NB500-179] - Lane view shows a specific band for TPX2 using HeLa cell lysate and antibody at 1:250. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly positive for both RHAMM and the intracellular positive control protein TPX2 (right panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0073548) licensed under a CC-BY licence.
