CABT-RM172

antibody from Creative Diagnostics

Targeting: TERF1 PIN2, TRBF1, TRF, TRF1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

CABT-RM172

Provider

Creative Diagnostics

Product name

Rat Anti-TERF1 monoclonal antibody, clone 683D

Antibody type

Monoclonal

Description

Telomeric repeat-binding factor 1 is encoded by the Terf1 gene in murine species. TRF1 is a component of the shelterin complex that is involved in the regulation of telomere length and protection. It binds to telomeric DNA as a homodimer and protects telomeres. It is reported to bind to the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. TRF1 is shown to be regulated by the PI3K signaling pathway and AKT is shown to phosphorylate TRF1 at threonine 248 and 330 and serine 344. Inhibition of PI3K and AKT are known to result in decreased TRF1 levels and decreased TRF1 telomeric foci. TRF1 levels can also be regulated at the transcriptional level by Oct4 that binds to TRF1 promoter, which is shown to be sufficient to upregulate TRF1 mRNA and protein levels during in vitro generation of induced pluripotent stem cells. ADP-ribosylation of human TRF1 by tankyrase 1 releases TRF1 from telomeres promoting its ubiquitination and proteasome-mediated degradation.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG