Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-28423 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
Submitted references R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage.
Chatzidoukaki O, Stratigi K, Goulielmaki E, Niotis G, Akalestou-Clocher A, Gkirtzimanaki K, Zafeiropoulos A, Altmüller J, Topalis P, Garinis GA
Science advances 2021 Nov 19;7(47):eabj5769
Science advances 2021 Nov 19;7(47):eabj5769
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRF1 using 30 µg of A) A431 (B) H1299 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TRF1 polyclonal antibody (Product # PA5-28423) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRF1 Polyclonal Antibody (Product # PA5-28423). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRF1 Polyclonal Antibody (Product # PA5-28423) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TRF1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TRF1 Polyclonal Antibody (Product # PA5-28423) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRF1 Polyclonal Antibody detects TRF1 protein at spindle by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRF1 stained by TRF1 Polyclonal Antibody (Product # PA5-28423) diluted at 1:500. Blue: Fluoroshield with DAPI .