11515-1-AP

antibody from Proteintech Group

Targeting: NCAPH BRRN1, CAP-H, hCAP-H

Provider product page for 11515-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [19]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11515-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11515-1-AP, RRID:AB_2150003
Product name: NCAPH antibody
Antibody type: Polyclonal
Description: KD/KO validated NCAPH antibody (Cat. #11515-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NCAPH protein structure - 11515-1-AP shown in red.

Submitted references Genome-wide DNA supercoiling arises from transcription and SMC activity and mediates transcriptional negative feedback.

Zhu L, Yao Q, Vemuri C, Chen C
bioRxiv : the preprint server for biology 2026 Mar 26;

NCAPH promotes immune evasion via inhibiting PD-L1 protein degradation in head and neck squamous cell carcinoma.

Liu B, Xiang X, Cheng Y, Fei J, Wu M, Qu L, Zhao X, Chen X, Li Y, Du J, Mu D, Zhai H, Shen Q, Chen Y, Yang C
Cancer letters 2026 Feb 28;639:218218

NCAPH Promotes the Proliferation of Prostate Cancer Cells Via Modulating the E2F1 Mediated PI3K/AKT/mTOR Axis.

Shi Q, Ma J, Pan X, Liu T, Chen K, Xu M, Wu Z, Sun B, Zhang M, Luo Y
International journal of medical sciences 2025;22(4):940-954

MiR-1976 affects lung squamous cell carcinoma development by targeting NCAPH.

Li Y, Zhou H, Yao Y, Fan Z
Journal of applied genetics 2025 Jul 23;

NCAPH serves as a prognostic factor and promotes the tumor progression in glioma through PI3K/AKT signaling pathway.

Liang J, Yun D, Jin W, Fan J, Wang X, Wang X, Li Y, Yu S, Zhang C, Li T, Yang X
Molecular and cellular biochemistry 2025 Jan;480(1):589-605

Non-SMC condensin I complex subunit H promotes cell proliferation and inhibits cell apoptosis of clear cell renal cell carcinoma by activating the PI3K/AKT pathway.

Ha H, Wang J, Zhang X, Du Y, Xiong W, Li S, Shang P
Translational andrology and urology 2025 Aug 30;14(8):2153-2170

ZCCHC3 and Efp coordinately contribute to the pathophysiology of triple-negative breast cancer by modulating NCAPH.

Fujimoto A, Ikeda K, Takeiwa T, Osaki A, Horie K, Inoue S
Biochemical and biophysical research communications 2024 Nov 26;735:150663

Proximity labelling of pro-interleukin-1α reveals evolutionary conserved nuclear interactions.

Wellens R, Tapia VS, Seoane PI, Bennett H, Adamson A, Coutts G, Rivers-Auty J, Lowe M, Green JP, Lopez-Castejon G, Brough D, Hoyle C
Nature communications 2024 Aug 8;15(1):6750

NCAPH, ubiquitinated by TRIM21, promotes cell proliferation by inhibiting autophagy of cervical cancer through AKT/mTOR dependent signaling.

Wang S, Qiao X, Cui Y, Liu L, Cooper T, Hu Y, Lin J, Liu H, Wang M, Hayball J, Wang X
Cell death & disease 2024 Aug 6;15(8):565

Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes.

Hibino K, Sakai Y, Tamura S, Takagi M, Minami K, Natsume T, Shimazoe MA, Kanemaki MT, Imamoto N, Maeshima K
Nature communications 2024 Aug 21;15(1):7152

StayGold variants for molecular fusion and membrane-targeting applications.

Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A
Nature methods 2024 Apr;21(4):648-656

Loss-of-function variants in KCTD19 cause non-obstructive azoospermia in humans.

Liu J, Rahim F, Zhou J, Fan S, Jiang H, Yu C, Chen J, Xu J, Yang G, Shah W, Zubair M, Khan A, Li Y, Shah B, Zhao D, Iqbal F, Jiang X, Guo T, Xu P, Xu B, Wu L, Ma H, Zhang Y, Zhang H, Shi Q
iScience 2023 Jul 21;26(7):107193

NCAPH regulates gastric cancer progression through DNA damage response.

Wang Y, Li JQ, Yang ZL, Wang L, Zhang JC, Sun YF, Li ZY, Qin L
Neoplasma 2022 Mar;69(2):283-291

NCAPH is a prognostic biomarker and associated with immune infiltrates in lung adenocarcinoma.

Li C, Meng J, Zhang T
Scientific reports 2022 Jun 10;12(1):9578

NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway.

Li B, Xiao Q, Shan L, Song Y
Cell cycle (Georgetown, Tex.) 2022 Feb;21(4):427-438

OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH.

Ogura T, Azuma K, Sato J, Kinowaki K, Takayama KI, Takeiwa T, Kawabata H, Inoue S
International journal of molecular sciences 2021 Oct 25;22(21)

Non-SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma.

Sun C, Huang S, Wang H, Xie R, Zhang L, Zhou Q, He X, Ju W
Molecular carcinogenesis 2019 Dec;58(12):2266-2275

Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.

Takagi M, Ono T, Natsume T, Sakamoto C, Nakao M, Saitoh N, Kanemaki MT, Hirano T, Imamoto N
Journal of cell science 2018 Mar 22;131(6)

NCAPH plays important roles in human colon cancer.

Yin L, Jiang LP, Shen QS, Xiong QX, Zhuo X, Zhang LL, Yu HJ, Guo X, Luo Y, Dong J, Kong QP, Yang CP, Chen YB
Cell death & disease 2017 Mar 16;8(3):e2680

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 11515-1-AP(NCAPH antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using NCAPH antibody 11515-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-NCAPH (IP:11515-1-AP, 3ug; Detection:11515-1-AP 1:800) with HeLa cells lysate 3000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 11515-1-AP(NCAPH antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue