Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-51727 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCAPH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LHCQDYRSEL LFPSDVQTLS TGEPLELPEL GCVEMTDLKA PLQQCAEDRQ ICPSLAGFQF TQWDSETHNE SVSALVDKFK KNDQVFDINA EVDESDCGDF PDGSLGDDFD ANDEPDHT Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 82%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NCAPH in human cell lines U2OS and SK-MEL-30 using a NCAPH Polyclonal Antibody (Product # PA5-51727). Corresponding NCAPH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human testis using a NCAPH Polyclonal Antibody (Product # PA5-51727) shows moderate cytoplasmic positivity in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human kidney, skin, testis and tonsil using NCAPH Polyclonal Antibody (Product # PA5-51727) (A) shows similar protein distribution across tissues to an independent NCAPH Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human kidney using NCAPH Polyclonal Antibody (Product # PA5-51727) shows no positivity as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human testis using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human skin using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in epidermal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NCAPH in human tonsil using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in germinal center cells.