PA5-51727

antibody from Invitrogen Antibodies

Targeting: NCAPH BRRN1, CAP-H, hCAP-H

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51727

Provider

Invitrogen Antibodies

Product name

NCAPH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LHCQDYRSEL LFPSDVQTLS TGEPLELPEL GCVEMTDLKA PLQQCAEDRQ ICPSLAGFQF TQWDSETHNE SVSALVDKFK KNDQVFDINA EVDESDCGDF PDGSLGDDFD ANDEPDHT

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NCAPH in human cell lines U2OS and SK-MEL-30 using a NCAPH Polyclonal Antibody (Product # PA5-51727). Corresponding NCAPH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human testis using a NCAPH Polyclonal Antibody (Product # PA5-51727) shows moderate cytoplasmic positivity in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human kidney, skin, testis and tonsil using NCAPH Polyclonal Antibody (Product # PA5-51727) (A) shows similar protein distribution across tissues to an independent NCAPH Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human kidney using NCAPH Polyclonal Antibody (Product # PA5-51727) shows no positivity as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human testis using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human skin using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in epidermal cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCAPH in human tonsil using NCAPH Polyclonal Antibody (Product # PA5-51727) shows nuclear positivity in germinal center cells.