Explore
Validate
Learn
Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C815384 - Provider product page
- Provider
- LSBio
- Product name
- NCAPH / CAP-H Antibody (Preservative Free) LS-C815384
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NCAPH rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 82 kDa. Observed band size: 110 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCAPH was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-NCAPH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 110 kDa.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NCAPH was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-NCAPH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 110 kDa.
