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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX120402 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX120402, RRID:AB_10721038
- Product name
- DSN1 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment.
KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Milev MP, Hasaj B, Saint-Dic D, Snounou S, Zhao Q, Sacher M
The Journal of cell biology 2015 Apr 27;209(2):221-34
The Journal of cell biology 2015 Apr 27;209(2):221-34
KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Caldas GV, DeLuca KF, DeLuca JG
The Journal of cell biology 2013 Dec 23;203(6):957-69
The Journal of cell biology 2013 Dec 23;203(6):957-69
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with DSN1 antibody [N3C3] (GTX120402) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 2% PFA for IF staining. Primary Ab diluted at 1: 1000 with 10% normal donkey serum in PHEM buffer.ACA is used as a marker for inner kinetochore.(Image kindly provided by Drs. J. Herman and J. DeLuca, Colorado State University)
