Explore
Validate
LearnPA5-34879
antibody from Invitrogen Antibodies
Targeting: DSN1
C20orf172, dJ469A13.2, hKNL-3, KNL3, MIS13
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34879 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DSN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Hela. Predicted reactivity: Pig (87%), Rhesus Monkey (94%), Bovine (82%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DSN1 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a DSN1 Polyclonal Antibody (Product # PA5-34879) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DSN1 in HeLa cells fixed with 2% PFA using a DSN1 polyclonal antibody (Product # PA5-34879) at a dilution of 1:1000 with 10% normal donkey serum in PHEM buffer. ACA is used as a marker for inner kinetochore.
