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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- 613102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613102, RRID:AB_2201135
- Product name
- Purified anti-Elongin C
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length
- Description
- Elongin C (also known as RNA polymerase II transcription factor SIII p15 subunit and transcription elongation factor B polypeptide 1) is a 12.5 kD member of the SKP1 family. Elongin functions as a regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. The Poly6131 antibody recognizes the human and mouse elongin C protein and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.
GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA.
Mao X, Gluck N, Chen B, Starokadomskyy P, Li H, Maine GN, Burstein E
The Journal of biological chemistry 2011 Sep 16;286(37):32355-65
The Journal of biological chemistry 2011 Sep 16;286(37):32355-65
GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA.
Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, Burstein E
Genes & development 2009 Apr 1;23(7):849-61
Genes & development 2009 Apr 1;23(7):849-61
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Elongin C antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
