Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations [0]
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- Product number
- 14-9928-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MD-2 Monoclonal Antibody (9B4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 9B4 antibody reacts with human MD-2, an approximately 30 kDa molecule, which physically associates with the extracellular domain of TLR4. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4 since in MD-2(-/-) embryonic fibroblasts, TLR4 was not able to reach the plasma membrane and predominantly resided in the Golgi apparatus, whereas TLR4 was distributed at the leading edge surface of cells in wild-type embryonic fibroblasts. MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella typhimurium infection. Applications Reported: The 9B4 antibody has been reported for use in immunoprecipitation. Applications Tested: This 9B4 antibody has been tested by immunoprecipitation of recombinant MD-2 from cell supernatant. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9B4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references The lipopolysaccharide core of Brucella abortus acts as a shield against innate immunity recognition.
Lipopolysaccharide (LPS)-binding protein inhibits responses to cell-bound LPS.
MD-2 binds to bacterial lipopolysaccharide.
Conde-Álvarez R, Arce-Gorvel V, Iriarte M, Manček-Keber M, Barquero-Calvo E, Palacios-Chaves L, Chacón-Díaz C, Chaves-Olarte E, Martirosyan A, von Bargen K, Grilló MJ, Jerala R, Brandenburg K, Llobet E, Bengoechea JA, Moreno E, Moriyón I, Gorvel JP
PLoS pathogens 2012;8(5):e1002675
PLoS pathogens 2012;8(5):e1002675
Lipopolysaccharide (LPS)-binding protein inhibits responses to cell-bound LPS.
Thompson PA, Tobias PS, Viriyakosol S, Kirkland TN, Kitchens RL
The Journal of biological chemistry 2003 Aug 1;278(31):28367-71
The Journal of biological chemistry 2003 Aug 1;278(31):28367-71
MD-2 binds to bacterial lipopolysaccharide.
Viriyakosol S, Tobias PS, Kitchens RL, Kirkland TN
The Journal of biological chemistry 2001 Oct 12;276(41):38044-51
The Journal of biological chemistry 2001 Oct 12;276(41):38044-51
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