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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-23137 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MD-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: human spleen or Ramos.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MD2 in A) human, B) mouse, and C) rat spleen lysate using a MD-2 polyclonal antibody (Product # PA5-23137) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MD-2 in human colon and human spleen. Samples were incubated in MD-2 polyclonal antibody (Product # PA5-23137) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 µm PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MD-2 in human brain tissue. Samples were incubated in MD-2 polyclonal antibody (Product # PA5-23137) using a dilution of 10 µg/mL. Isotype control antibody (top left) and MD2 antibody (bottom left, right).
