Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB100-56655 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56655, RRID:AB_838565
- Product name
- Rabbit Polyclonal MD-2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Cathepsin E in neutrophils contributes to the generation of neuropathic pain in experimental autoimmune encephalomyelitis.
IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils.
Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling.
MD-2 is required for disulfide HMGB1-dependent TLR4 signaling.
Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo.
Lipopolysaccharides and trophic factors regulate the LPS receptor complex in nodose and trigeminal neurons.
Harada Y, Zhang J, Imari K, Yamasaki R, Ni J, Wu Z, Yamamoto K, Kira JI, Nakanishi H, Hayashi Y
Pain 2019 Sep;160(9):2050-2062
Pain 2019 Sep;160(9):2050-2062
IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils.
Ellison MA, Gearheart CM, Porter CC, Ambruso DR
PloS one 2017;12(10):e0185956
PloS one 2017;12(10):e0185956
Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling.
Xiong X, Liu Y, Mei Y, Peng J, Wang Z, Kong B, Zhong P, Xiong L, Quan D, Li Q, Wang G, Huang H
Scientific reports 2017 Feb 6;7:41857
Scientific reports 2017 Feb 6;7:41857
MD-2 is required for disulfide HMGB1-dependent TLR4 signaling.
Yang H, Wang H, Ju Z, Ragab AA, Lundbäck P, Long W, Valdes-Ferrer SI, He M, Pribis JP, Li J, Lu B, Gero D, Szabo C, Antoine DJ, Harris HE, Golenbock DT, Meng J, Roth J, Chavan SS, Andersson U, Billiar TR, Tracey KJ, Al-Abed Y
The Journal of experimental medicine 2015 Jan 12;212(1):5-14
The Journal of experimental medicine 2015 Jan 12;212(1):5-14
Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo.
Martin I, Cabán-Hernández K, Figueroa-Santiago O, Espino AM
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3924-36
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3924-36
Lipopolysaccharides and trophic factors regulate the LPS receptor complex in nodose and trigeminal neurons.
Kunda PE, Cavicchia JC, Acosta CG
Neuroscience 2014 Nov 7;280:60-72
Neuroscience 2014 Nov 7;280:60-72
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MD-2 Antibody [NB100-56655] - Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-MD2 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY licence. anti-TLR4 (Novus Biological NBP2-24538).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MD-2 Antibody [NB100-56655] - Staining of normal human brain using control (rabbit Ig) at 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MD-2 Antibody [NB100-56655] - Staining of normal human brain using 100-56655 (MD-2)at 10 ug/ml.