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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- GTX120264 - Provider product page
- Provider
- GeneTex
- Product name
- MAFF antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- MAFF antibody detects MAFF protein by Western blot analysis.A. 30 ?g Huh7 whole cell lysate/extract B. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEMAFF antibody (GTX120264) dilution: 1:2000
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- GeneTex (provider)
- Main image
- Experimental details
- MAFF antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted by 1:20000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- A431 whole cell and nuclear extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MAFF antibody detects MAFF protein at cytoplasm and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MAFF antibody (GTX120264) diluted at 1:250.
