Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-35918 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAFF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, A431 nuclear extract, human MAFF-transfected 293T. Predicted reactivity: Mouse (94%), Rat (96%), Chicken (80%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAFF was performed by separating 30 µg of A431 whole cell and nuclear extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFF Polyclonal Antibody (Product # PA5-35918) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFF Polyclonal Antibody detects MAFF protein by western blot analysis.293T mock transfected (-) or transfected with a construct expressing human MAFF (+).12 % SDS-PAGE. MAFF Polyclonal Antibody (Product # PA5-35918) dilution: 1:20,000.