PA5-85462

antibody from Invitrogen Antibodies

Targeting: MAFF hMafF

Antibody data

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Validation data

Product number: PA5-85462 - Provider product page
Provider: Invitrogen Antibodies
Product name: MAFF Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Keep as concentrated solution. Predicted reactivity: Mouse (97%), Rat (97%), Chicken (86%), Bovine (99%). Positive Control: A431, A431 nuclear extract, rat skin, Huh7, HepG2, human MAFF-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MAFF protein structure - PA5-85462 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MAFF in A) Huh7 whole cell lysate, B) HepG2 whole cell lysate using MAFF polyclonal antibody (Product # PA5-85462) using 30 µg of sample at a dilution of 1:2000. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MAFF Polyclonal Antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.12 % SDS-PAGE. MAFF Polyclonal Antibody (Product # PA5-85462) dilution: 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MAFF in non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts using MAFF polyclonal antibody (Product # PA5-85462) using 30 µg of sample at a dilution of 1:20000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of MAFF was performed by separating 30 µg of A431 whole cell and nuclear extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFF Polyclonal Antibody (Product # PA5-85462) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MAFF Polyclonal Antibody detects MAFF protein by Western blot analysis. A. 30 µg Huh7 whole cell lysate/extract. B. 30 µg HepG2 whole cell lysate/extract.12 % SDS-PAGE. MAFF Polyclonal Antibody (Product # PA5-85462) dilution: 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MAFF was performed by separating 50 µg of rat tissue extract by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a MAFF Polyclonal Antibody (Product # PA5-85462) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MAFF Polyclonal Antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF Polyclonal Antibody (Product # PA5-85462) diluted by 1:20,000.