PA5-20932
antibody from Invitrogen Antibodies
Targeting: TMEM184B
C22orf5, DKFZP586A1024, FM08, HS5O6A
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-20932 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM184B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat lung tissue lysate. PA5-20932 can be used with blocking peptide PEP-1046.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references MicroRNA expression signature of oral squamous cell carcinoma: functional role of microRNA-26a/b in the modulation of novel cancer pathways.
Fukumoto I, Hanazawa T, Kinoshita T, Kikkawa N, Koshizuka K, Goto Y, Nishikawa R, Chiyomaru T, Enokida H, Nakagawa M, Okamoto Y, Seki N
British journal of cancer 2015 Mar 3;112(5):891-900
British journal of cancer 2015 Mar 3;112(5):891-900
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat lung tissue lysate using a TMEM184B polyclonal antibody (Product # PA5-20932) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TMEM184B in rat lung tissue lysate with TMEM184B Polyclonal Antibody (Product # PA5-20932) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human lung tissue using a TMEM184B polyclonal antibody (Product # PA5-20932) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TMEM184B in human lung tissue with TMEM184B Polyclonal Antibody (Product # PA5-20932) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of TMEM184B in human lung tissue with TMEM184B Polyclonal Antibody (Product # PA5-20932) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 TMEM184B expression was directly regulated by miR-26a/b in OSCC cell lines. ( A ) TMEM184B mRNA expression 72 h after transfection with miR-26a/b . GUSB expression was used for normalisation. ( B ) TMEM184B protein expression 72 h after transfection with miR-26a/b . GAPDH was used as a loading control. ( C ) The miR-26a/b binding site in the 3'-UTR of TMEM184B mRNA. Luciferase reporter assays were performed using vectors that included (WT) or lacked (DEL) the wild-type sequences of the putative miR-26a/b target site. Renilla luciferase assays were normalised to firefly luciferase values. * P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of si- TMEM184B transfection on OSCC cell lines. ( A ) TMEM184B mRNA expression levels were measured by RT-PCR 72 h after transfection with 10 nM si- TMEM184B . GUSB was used for normalisation. ( B ) TMEM184B protein expression 72 h after transfection with si- TMEM184B . GAPDH was used as a loading control. ( C ) Cell proliferation was determined by XTT assay 72 h after transfection with 10 nM si- TMEM184B . ( D ) Cell migration was determined by migration assay 48 h after transfection with 10 nM si- TMEM184B . ( E ) Cell invasion was determined by Matrigel invasion assay 48 h after transfection with 10 nM si- TMEM184B . * P