PAB29402

antibody from Abnova Corporation

Targeting: FBXO44 FBG3, FBX30, Fbx44, Fbxo6a, MGC14140

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29402

Provider

Abnova Corporation

Product name

FBXO44 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human FBXO44.

Antigen sequence

SLHRNLLHNPCAEEGFEFWSLDVNGGDEWKVEDLS
RDQRKEFPNDQVRSQARLRVQVPAVRSAPVVRARA
SGDLPARPGDHPAEERCQVEGGLPHILQLP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with FBXO44 polyclonal antibody (Cat # PAB29402) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with FBXO44 polyclonal antibody (Cat # PAB29402) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex with FBXO44 polyclonal antibody (Cat # PAB29402) shows strong nuclear and cytoplasmic positivity in neuronal cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)