Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-84728 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84728, RRID:AB_11024209
- Product name
- Rabbit Polyclonal FBXO44 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human FBXO44 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex.
The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.
Sjögren B, Swaney S, Neubig RR
PloS one 2015;10(5):e0123581
PloS one 2015;10(5):e0123581
The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.
Lu Y, Li J, Cheng D, Parameswaran B, Zhang S, Jiang Z, Yew PR, Peng J, Ye Q, Hu Y
The Journal of biological chemistry 2012 Nov 30;287(49):41014-22
The Journal of biological chemistry 2012 Nov 30;287(49):41014-22
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FBXO44 Antibody [NBP1-84728] - Analysis in control (vector only transfected HEK293T lysate) and FBXO44 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FBXO44 Antibody [NBP1-84728] - Analysis in human cell line SK-MEL-30.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human brain shows strong nuclear and cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FBXO44 Antibody [NBP1-84728] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
