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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- ABIN1881669 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Polymerase (DNA Directed) kappa (POLK) (Center), (AA 531-560) antibody
- Antibody type
- Polyclonal
- Antigen
- This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
- Description
- This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Center,AA 531-560
- Antibody clone number
- RB41936
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
Submitted references Critical amino acids in human DNA polymerases eta and kappa involved in erroneous incorporation of oxidized nucleotides.
Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially catalyzes extension reaction from the 3'-dC terminus.
Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct.
Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass.
Katafuchi A, Sassa A, Niimi N, Grúz P, Fujimoto H, Masutani C, Hanaoka F, Ohta T, Nohmi T
Nucleic acids research 2010 Jan;38(3):859-67
Nucleic acids research 2010 Jan;38(3):859-67
Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially catalyzes extension reaction from the 3'-dC terminus.
Fukuda H, Takamura-Enya T, Masuda Y, Nohmi T, Seki C, Kamiya K, Sugimura T, Masutani C, Hanaoka F, Nakagama H
The Journal of biological chemistry 2009 Sep 18;284(38):25585-92
The Journal of biological chemistry 2009 Sep 18;284(38):25585-92
Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct.
Irimia A, Eoff RL, Guengerich FP, Egli M
The Journal of biological chemistry 2009 Aug 14;284(33):22467-80
The Journal of biological chemistry 2009 Aug 14;284(33):22467-80
Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass.
Lone S, Townson SA, Uljon SN, Johnson RE, Brahma A, Nair DT, Prakash S, Prakash L, Aggarwal AK
Molecular cell 2007 Feb 23;25(4):601-14
Molecular cell 2007 Feb 23;25(4):601-14
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- POLK Antibody (Center) (ABIN1881669) western blot analysis in 293 cell line lysates (35 μg/lane). This demonstrates the POLK antibody detected the POLK protein (arrow).
